In silico cloning of new endothelial-specific genes
نویسندگان
چکیده
منابع مشابه
Cellulose synthesis: Cloning in silico
Cell walls are of interest to plant biochemists and cell biologists for two main reasons: they regulate cellular morphogenesis and act as structural support materials. As it is a major component of plant cell walls and a natural product of considerable commercial value, cellulose has long been a focus of interest in cell-wall biology, although it is far from the only constituent of cell walls. ...
متن کاملIn silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors
Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...
متن کاملin silico designing of a new cysteine analogue of hirudin variant 3 for site specific pegylation
hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. recombinant hirudin (r-hir) displays certain drawbacks including bleeding and immunogenicity. to solve these problems, cysteine-specific pegylation has been proposed as a successful technique. however, proper selection of the appropriate cysteine residue for substitution is a critical step. this study has, for the ...
متن کاملCloning and expression of genes encoding human pregnancy-specific glycoproteins.
The pregnancy-specific glycoproteins (PSGs) of the human placenta and the carcinoembryonic antigens comprise a subfamily within the immunoglobulin superfamily. There may be as many as 20 different PSG genes which are predominantly expressed in the placenta. As an initial step toward understanding the control of PSG expression, we isolated and characterized two nearly identical PSG genes, PSG1 a...
متن کاملDesign, simplified cloning, and in-silico analysis of multisite small interfering RNA-targeting cassettes
Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Genetics
سال: 2001
ISSN: 1061-4036,1546-1718
DOI: 10.1038/87128